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Polygence Scholar2022
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Maya Srinivasan

Class of 2024San Jose, California


Project: Examining visual stimulated behavior in ChR2 and "Moonwalker" Drosophila Melanogaster strains


  • "Testing the Neuronal Response with Different Optogenetic Tools" with mentor Oscar (Working project)

Project Portfolio

Testing the Neuronal Response with Different Optogenetic Tools

Started Oct. 7, 2022

Abstract or project description

Drosophila melanogaster optogenetic tools are used to map neural systems of vision, instinct, and reactive behavior. Bilateral pair Moonwalker Descending Neurons (MDNs) are just one type of neuron that has been identified as part of the instinctual backward walking circuit, which leads to questions about the pathways of such behaviors. While backward walking and other similar behaviors are still neurologically mysterious, tools like CsChrimson and ChR2 can help us classify neuroanatomical connections. In this study, we aim to identify the similarities between transgenic UAS- CsChrimson VT050660-GAL4 ‘moonwalker’ and UAS-Channelrhodopsin2 (ChR2) ‘seizure’ strains, through optogenetic stimulation. We used red and blue light-activated channels to drive either backward walking(moonwalked) or pain-less seizure(seizure) behavior and recorded patterns in behavior both during and after assays. Previous experimentation has identified stalling after “moonwalker” red light activation, hypothetically due to a sudden drop in neurotransmitter release. However, the corroboration of activated behavior in both strains could reveal key neuroanatomical pathways, furthering the classification of Drosophila melanogaster structure.