Sakina Rizvi
Class of 2026Redmond, Washington
About
Hi! My name is Sakina and my Polygence Project regards the disadvantages of CRISPR, the new genome editing technology that is proving to be increasingly successful yet has flaws leading to unpredictable side effects. After my project is complete, I would like to hibernate for at least a week (just kidding), I mean, learn more about genetics and the biotechnology field.Projects
- "How can the foundational elements of CRISPR-Cas9 genome editing technology, such as different PAM sequences and DNA repair pathways, be adjusted to improve efficiency and specificity while reducing off-target effects?" with mentor Samantha (Oct. 30, 2024)
Project Portfolio
How can the foundational elements of CRISPR-Cas9 genome editing technology, such as different PAM sequences and DNA repair pathways, be adjusted to improve efficiency and specificity while reducing off-target effects?
Started Oct. 16, 2024
Abstract or project description
CRISPR-Cas9 has revolutionized genome editing by offering a versatile tool to precisely modify DNA. However, challenges such as off-target effects, limited efficiency, and ethical concerns persist. Researchers are addressing these issues through diverse strategies, including chromatin-modifying agents, engineered Cas9 variants, and improved delivery methods like mRNA and ribonucleoprotein complexes. Base editing techniques using deaminases, such as Cytosine Base Editors, further enhance editing accuracy by reducing the risk of unintended genetic changes. Despite its transformative potential in medicine and agriculture, CRISPR’s application raises ethical concerns, particularly in germline editing and genetically modified organisms. Regulatory frameworks established by organizations like the FDA, NIH, and WHO are essential for ensuring public safety and ethical integrity. Key advancements led by pioneering research groups and ongoing trials continue to refine the technology, showcasing its promise in treating genetic disorders and enhancing crop traits. CRISPR’s future lies in balancing innovation with responsible application, addressing technical challenges, and adhering to rigorous ethical guidelines to maximize its societal benefits.