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Uses of CRISPR/Cas9 gene editing in chimeric antigen receptor T cell therapy to treat B-cell acute lymphoblastic leukemia

Project by Polygence alum Elena

Uses of CRISPR/Cas9 gene editing in chimeric antigen receptor T cell therapy to treat B-cell acute lymphoblastic leukemia

Project's result

Published in the Journal of High School Science

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Summary

B-cell acute lymphoblastic leukemia (B-ALL) is a hematological malignancy that affects almost 500,000 people per year, approximately two-thirds of them being children and adolescents. While current treatments such as chemotherapy and hematopoietic stem cell transplantation have significantly reduced the death rate of leukemia, there are still many obstacles to hurdle. Scientists have come up with therapies that aim to overcome these challenges, including chimeric antigen receptor (CAR) T cell therapy. CAR-T cell therapy is a relatively new treatment that aims to increase survival in relapsed/refractory B-ALL. This review paper discusses the mechanisms of chimeric antigen T cells and their current limitations. Next, it goes over the mechanistic details of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and the CRISPR associated (Cas)9 protein genome editing system and how it disrupts DNA, along with how it can be used to modify human cells. The applications of CRISPR/Cas9 to CAR-T cell therapy and how it can be used to solve current limitations are explored. CRISPR can improve CAR-T cells by limiting T cell exhaustion, limiting antigen escape, and preventing graft-vs-host disease. This paper also looks at ongoing clinical trials and discuss possible solutions in the future with respect to CAR-T cells’ use in B-cell leukemia. CAR-T cell therapy is a developing therapy that is viewed by many as the next stepping in B-ALL treatment, and its limitations can be overcome with the CRISPR/Cas9 gene editing complex. Using CRISPR to manufacture CAR-T cells has the potential to increase survival rates of leukemia dramatically.

Jacob

Jacob

Polygence mentor

PhD Doctor of Philosophy candidate

Subjects

Medicine, Biology

Expertise

Biology, Biochemistry, Genetics, Bioethics

Elena

Elena

Student

School

Mercer Island High School

Graduation Year

2024

Project review

“My mentor exceeded my expectations.”

About my mentor

“My mentor introduced me to the topic and guided me through writing my article. He knew exactly the right way to talk about my topic to me -- he didn't underestimate or overestimate my knowledge.”