Novi High SchoolClass of 2023
- Future Possibilities for the use of CRISPR on Mutations in Three Eye Disorders with mentor Kevin (Dec. 29, 2021)
Future Possibilities for the use of CRISPR on Mutations in Three Eye DisordersStarted Mar. 18, 2021
Abstract or project description
My research paper focuses on three eye disorders,X-linked Congenital Night Blindness, Snowflake Vitreoretinal Degeneration, and Cataract Microcornea syndrome, and their potential mutations that can be corrected using CRISPR technology. In this project, I initially spent time learning about CRISPR's structure and its current usage in the eye disorder Leber Congenital Amaurosis. I then applied this knowledge to determine which mutations fit in CRISPR requirements, and what mutations would have the most significant impact on treating the symptoms. Throughout this experience, I learned how to conduct research and write a formal paper. This experience improved my literature skill from editing and writing my paper, and further advanced my interest for Genetics. Abstract: CRISPR [Clustered Regularly Interspaced Short Palindromic Repeats] technology is a useful tool to insert, delete, and substitute DNA in the genome. This is done by separating DNA via double-stranded breaks and can be performed through two different mechanisms: homology-directed Repair [HDR] and Non-Homologous End Jointing [NHEJ]. Double strand breaks would be used for a tracrRNA:crRNA to guide the enzyme cas9 to insert, delete, or substitute the desired DNA. However, CRISPR has limitations on which DNA sequences it can work with. Other concerns include ethical questions and base-pair limitations. X-linked Congenital Night Blindness, Snowflake Vitreoretinal Degeneration, and Cataract Microcornea syndrome are three genetic diseases caused by mutations in the genes CACNA1F, KCNJ13, and ABCA3 respectively. This research paper will discuss the possibilities of CRISPR and its impact on correcting mutations for all three eye disorders. This paper deciphers specific substitutions that CRISPR can be used on, and the impact this correction will have on the three disorders.